RB-LD-1000 Pack Size: 6 ml

6X gel loading buffer is used as DNA tracking dye in agarose gel electrophoresis. The buffer consists of two dyes – Bromophenol Blue and Xylene cyanol FF. Bromophenol Blue migrates through agarose gels at approximately the same rate as linear ds DNA 300 bp in length whereas xylene cyanol FF migrates at approximately the same rate as linear ds DNA 4 kb in length. These relationships are not significantly affected by the concentration of agarose in the gel over the range of 0.5% – 1.4%.

1X Gel Loading Buffer: 0.04% Bromophenol Blue, 0.04% Xylene Cyanol FF, 2.5% Ficoll and 10 mM EDTA.

• Buffer supplied at 6X concentration.
• Working concentration is 0.5 – 1X.

Store: 4°C