BLUelf Prestained Protein Ladder

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BLUelf Prestained Protein Ladder

Short Description

Cat No : RB008-0600

The BLUelf Prestained Protein Ladder is a three-color protein standard with 13 prestained proteins covering a wide range molecular weights from 3.5 to 245 kilodalton (kDa). Proteins are covalently coupled with a blue chromophore except for two reference bands (one green and one red band at 25 kDa and 75 kDa respectively) when separated on SDS-polyacrylamide gel electrophoresis (SDSPAGE) with Tris-glycine-SDS running buffer. The BLUelf Prestained Protein Ladder is designed for monitoring protein separation during SDS-PAGE, verification of Western transfer efficiency on membranes (PVDF, nylon, or nitrocellulose) and for approximating the size of p roteins. The ladder is supplied in gel loading buffer and is ready to use. Do not heats, dilute, and add reducing agent before loading.


Features

  • Broad range: 3.5-245 kDa (Tris-glycine-SDS running buffer)
  • Ready-to-use: supplied in a loading buffer for direct loading on gels
  • Easy to identify: includes the ~25, ~75 kDa reference bands coupled with a green and a red dye
  • Sharp bands

Applications

  • Monitoring of protein migration during SDS-PAGE.
  • Monitoring of protein transfer onto membranes during Western blots.
  • Sizing of proteins on SDS-PAGE and Western blots.

Storage Buffer
Approximately 0.1~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 0.2 mM Dithiothreitol, 3.6 M Urea, and 15 % (v/v) Glycerol.


Quality Control
The quality of the BLUelf Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.


BLUelf Prestained Protein Ladder Protocol
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-PAGE gel:

  • 5 μl per well for mini-gels, 2.5 μl per well for blots
  • 10 μl per well for large gels, 5 μl per well for blots
  • Apply more for thicker (> 1.5 mm) or larger gel

Guide for Molecular Weight Estimation (kDa)
Migration patterns of BLUelf Prestained Protein Ladder in different electrophoresis conditions are listed below:

 

Note:
1. The apparent molecular weight of each protein has been determined by calibration against unstained protein standards
2. Supplemental data should be considered for more accurate adjustment in different electrophoresis conditions.
All products are for research use only.
Caution: Not intended for human or animal diagnostic or therapeutic uses.

About

Renwik Bioinnovations (RBI) Private Limited started by experienced young Biotechnologist to promote scientific culture in the field of biotechnology in India. 

It is our endeavor to Manufacturing of ingenious Instruments and tie-up with world’s leading suppliers who offer quality products with the aim of complete customer satisfaction.

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Support

For General Inquiry : info@renwikbio.com renwikbio@gmail.com

For Tech Support : support@renwikbio.com (Please provide your order number in the email)

U-41, Upadhyay Block, Shakarpur, New Delhi-110092 (India).

Privacy Policy Copyright ©2021 Renwik Bioinnovations Pvt. Ltd. All Rights Reserved.

All products are for Research Purpose Only. Not for use in diagnostic or therapeutic uses. Do not use in humans.

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