PCR Master Mix
◆ No post-staining processing of DNA required.
◆ No need to prepare PCR Reagents.
◆ Direct loading onto your agarose gel for analysis.
◆ Sensitivity – High degree of sensitivity as the ethidium bromide.
◆ Speed – No destaining requirement.
◆ Compatibility – Use the blue light or UV to detect the signal.
PCR Master Mix
Cat No: RB213-050 Size-50 rxn
Cat No: RB213-0100 Size-100 rxn
Cat No: RB213-0250 Size-250 rxn
Cat No: RB213-0500 Size-500 rxn
Renwik PCR Master Mix is a ready-to-use PCR reaction mixture. Simply add primers, template, and water, the reagent will execute primer extensions and other molecular biology applications. The OnePCR is a pre-mixed solution containing Taq DNA polymerase, PCR buffer, dNTPs, gel loading dyes, and fluorescence dye. OnePCR which contains the Taq DNA polymerase is purified from the E. coli., and expressing the Thermus aquaticus DNA polymerase gene. This enzyme has a 5′ → 3′ DNA polymerase and the 5′ → 3′ exonuclease activity but lacks the 3′ → 5′ exonuclease activity. The OnePCR, which contains the fluorescence dye, is directly detected on an LED transilluminator or UV epi-illuminator after the DNA electrophoresis. The OnePCR contains red tracking dyes, provides a safe, non-toxic, and non-mutagenic alternative to ethidium bromide for instantaneous band visualization, that is environmentally friendly containing no hazardous chemicals. The tracking dyes run at 10 bp on a 1% agarose gel. The OnePCR mixture is supplied at the 2X concentration to allow approximately 50% of the final reaction volume to be used for the addition of primer and template solutions. The reagents are provided with sufficient .amplification reactions of 20 μl each.
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