PiNK Plus Prestained Protein Ladder 10-175 Kda

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PiNK Plus Prestained Protein Ladder 10-175 Kda

Short Description

Cat No: RB005-0600

The PiNK Plus Prestained Protein Ladder contains 11 proteins that resolve into sharp, tight bands in the range of 10-175 kilodalton (kDa). The PiNK Plus Prestained Protein Ladder allows you to monitor molecular weight separation during on SDS-polyacrylamide gel electrophoresis (SDS-PAGE), estimate molecular weights of proteins of interest, and evaluate western transfer efficiency.
Applications
◆  Monitoring of protein migration during SDS-PAGE gel electrophoresis.
◆  Monitoring of protein transfer onto membranes during Western blots.
◆  Sizing of proteins on SDS-PAGE gels and Western blots.
Storage Buffer
Approximately 0.2~0.4 mg/ml of each protein in the buffer (20 mM Tris-phosphate, pH 7.5 at 25°C), 2 % SDS, 1 mM Dithiothreitol, 4.8 M Urea, and 12 % (v/v) Glycerol.
Quality Control
The quality of the PiNK Plus Prestained Protein Ladder is tested on a lot-to-lot basis to ensure consistent product quality.
Storage
Stable for up to 2 weeks at 25°C.
Stable for up to 3 months at 4°C.
For long term storage, store at -20°C.
PiNK Plus Prestained Protein Ladder Protocol
1. Thaw the ladder either at room temperature or at 37-40°C for a few minutes to dissolve precipitated solids. Do not boil.
2. Mix thoroughly to ensure the solution is homogeneous.
3. Load the following volumes of the ladder on SDS-PAGE gel:
◆  5 μl per well for mini-gels, 2.5 μl per well for blots
◆  10 μl per well for large gels, 5 μl per well for blots
◆  Apply more for thicker (> 1.5 mm) or larger gel
Guide for Molecular Weight Estimation (kDa)
Migration patterns of PiNK Plus Prestained Protein Ladder in different electrophoresis conditions are listed below:

About

Renwik Bioinnovations (RBI) Private Limited started by experienced young Biotechnologist to promote scientific culture in the field of biotechnology in India. 

It is our endeavor to Manufacturing of ingenious Instruments and tie-up with world’s leading suppliers who offer quality products with the aim of complete customer satisfaction.

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All products are for Research Purpose Only. Not for use in diagnostic or therapeutic uses. Do not use in humans.

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