Plasmid Miniprep Kit


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Plasmid Miniprep Kit

Short Description

Cat No : RB005-0100 Size : 100 rxns

The Plasmid miniPREP Kit provides a fast, simple, and cost-effective method for plasmid DNA isolation
from cultured bacterial cells. The Plasmid miniPREP Kit is based on the alkaline lysis of bacterial cells,
followed by the binding of the DNA onto the glass fiber matrix of the spin column in the presence of high
salt. The phenol extraction and ethanol precipitation are not required, and the high-quality plasmid DNA
is eluted in a small volume of Tris buffer (included in the kit) or water (pH between 7.0 and 8.5, not
provided in the kit). The plasmid DNA purified with the Plasmid miniPREP Kit is suitable for a variety of
routine applications including restriction enzyme digestion, sequencing, library screening, in vitro translation,
transfection of robust cells, ligation, and transformation. The entire procedure can be completed within
15-20 minutes.
 Ready-to-use DNA for high performance in any downstream application.
 Consistent DNA yields from a small amount of the starting material.
 Time flexibility.
 Quantity of DNA needed.  Molecular weight and size of DNA.
 The purity of DNA required


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