SYBR Green PCR Master Mix
SYBR Green PCR Master Mix
Everything you need for SYBR® Green dye-based PCR amplification and detection in a convenient, single-tube format. Applied Biosystems® SYBR® Green PCR Master Mix combines SYBR® Green I dye, AmpliTaq Gold® DNA Polymerase, dNTPs with dUTP, Passive Reference 1, and optimized buffer in the convenience of a single vial.
• Premixed components stored at 2–8°C significantly reduce assay setup time
• SYBR® Green I dye detects double-stranded DNA, so specific probes are not required
• AmpliTaq Gold® DNA polymerase minimizes nonspecific product formation to achieve superior performance
• dUTP significantly reduces carryover contamination when used in conjunction with uracil-DNA glycosylase
• Proprietary buffer enhancements ensure performance and reliability
Maximum Flexibility and Convenience
Applied Biosystems® SYBR® Green PCR Master Mix provides maximum flexibility at a reduced cost because no target-specific TaqMan® probes are required. SYBR® Green I dye is a double-stranded DNA binding dye that detects any double-stranded DNA generated during PCR. The hot-start enzyme AmpliTaq Gold® DNA Polymerase minimizes nonspecific product formation (including primer-dimers), yielding superior performance and sensitivity. Passive Internal Reference 1 is provided to normalize non-PCR–related fluorescence fluctuations. This minimizes well-to-well variability that can result from a variety of causes, such as pipetting error or sample evaporation. SYBR® Green I dye is ideal for target identification (screening assays) or when a limited number of assays is needed.
Try PowerUp SYBR Green Master Mix, our newest, high-performance, SYBR dye-based master mix for superior performance at a very competitive price. With PowerUp SYBR Green Master Mix, we’ve taken the best of SYBR Green PCR Master Mix and added additional capabilities for your gene expression analysis.
|For Use With (Equipment):||7000 System, 7300 System, 7500 System, 7700 System, 7900HT System, Applied Biosystems StepOnePlus™ Fast Real-Time PCR System, QuantStudio™, StepOne™, Standard Mode, StepOnePlus™, Standard Mode, ViiA™ 7 System|
|Label or Dye:||SYBR® Green|
|Passive Reference Dye:||ROX (Pre-mixed)|
|Primer Probe Compatibility:||Unlabeled Oligos|
|Quantity:||1 x 5mL|
|Sample Type:||DNA (Genomic), cDNA|
|Shipping Condition:||Wet Ice|
|Polymerase:||AmpliTaq Gold DNA Polymerase|
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